Composite

Part:BBa_K733009:Design

Designed by: WANG, Yuqi   Group: iGEM12_HKUST-Hong_Kong   (2012-09-16)

Ptms+BBa_E0240: Ptms+RBS+GFP+Double


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 736


Design Notes

None.

Source

We digest and ligate our Ptms and BBa_E0240 from 2012 Kit Plate.

Backbone: Ptms in pSB1C3. Enzyme used: SpeI and PstI-HF.

Insert: BBa_E0240. Enzyme used: XbaI and PstI-HF. Purified by gel purification.

References